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Mechanisms of miRNA Expression & Function


Chipman LB, Pasquinelli AE. miRNA Targeting: Growing beyond the Seed. Trends Genet. 2019 Jan 9. pii: S0168-9525(18)30224-5. doi: 10.1016/j.tig.2018.12. 005 Review. PMCID: PMC7083087

Schreiner WP and Pasquinelli AE. Making and Maintaining MicroRNAs in Animals, Chapter for “Essentials of microRNAs in neurogenesis: ontogenesis and plasticity of the mammalian neural system” 1st Ed. De Pietri Tonelli, Elsevier, 2017. p1-17.

Broughton JP, Pasquinelli AE. A tale of two sequences: microRNA-target chimeric reads. Genet Sel Evol. 2016 Apr 4;48:31. doi: 10.1186/s12711-016-0209-x. Review. PMCID: PMC4819279

Pasquinelli AE. MicroRNAs: heralds of the non-coding RNA revolution. RNA, 2015. 21(4):709-710. PMCID: PMC4371344

Van Wynsberghe PM, Pasquinelli AE. Period Homolog LIN-42 regulates miRNA transcription to impact developmental timing. Worm, 2014 3(4)e974453. PMCID: PMC4589994

Lima SA, Pasquinelli AE. Identification of miRNAs and their targets in C. elegans. Adv Exp Med Biol. 2014; 825:431-50. DOI: 10.1007/978-1-4939-1221-6_12

Pasquinelli AE. The primary target of let-7 miRNA. Biochem. Soc. Trans. 2013 Aug 1;41(4):821-4. DOI: 10.1042/BST20130020

Massirer KB, Pasquinelli AE.  MicroRNAs that interfere with RNAi. Worm, 2013 Jan/Feb/Mar;2(1):e1-6. PMCID: PMC3670461

Finnegan EF, Pasquinelli AE.  MicroRNA biogenesis: Regulating the regulators. Critical Reviews In Biochemistry & Molecular Biology, 2013 Jan-Feb;48(1):51-68. PMCID: PMC3557704

Aalto AP, Pasquinelli AE. Small non-coding RNAs mount a silent revolution in gene expression. Current Opinion in Cell Biology, 2012 Jun;24(3):333-40. PMCID: PMC3372702

Pasquinelli AE. MicroRNAs and their targets: recognition, regulation and an emerging reciprocal relationship.Nat Rev Genet. 2012 Mar 13;13(4):271-82. DOI: 10.1038/nrg3162

Mondol V, Pasquinelli AE. Let’s make it happen:  The role of let-7 microRNA in development. In Eran Hornstein, editors: MicroRNAs in Development, CTDB, UK: Academic Press, 2012, 99:1-30. DOI: 10.1016/B978-0-12-387038-4.00001-X

Kai ZS, Pasquinelli AE. MicroRNA assassins: factors that regulate the disappearance of miRNAs. Nat Struct Mol Biol. 2010 Jan;17(1):5-10. Review. PMCID: PMC6417416

Massirer K, Pasquinelli AE. The evolving role of microRNAs in animal gene expression. BioEssays 2006 28:449-452. DOI: 10.1002/bies.20406

Bagga S, Pasquinelli AE. Identification and analysis of microRNAs. Genet Eng (N Y) 2006 27:1-20. DOI: 10.1007/0-387-25856-6_1

Pasquinelli AE, Hunter SE, Bracht J. MicroRNAs:  A Developing Story. Curr. Opinion in Cell and Devel. Biol. 2005 15:200-205. DOI: 10.1016/j.gde.2005.01.002

Pasquinelli AE. MicroRNAs:  a Small Contribution from Worms.  In RNA Interference Technology: From Basic Science to Drug Development, ed. K Appasani. Cambridge, UK: Cambridge University Press. 2005. p69-83.

Pasquinelli AE. MicroRNAs:  Deviants No Longer. Trends in Genetics.  2002 18:171-173.DOI: 10.1016/s0168-9525(01)02624-5


Primary Research

Broughton JP, Pasquinelli AE. Detection of microRNA-Target Interactions by Chimera PCR (ChimP).Methods Mol Biol. 2018;1823:153-165. DOI: 10.1007/978-1-4939-8624-8_12

Broughton JP, Lovci MT, Huang JL, Yeo GW, Pasquinelli AE. Pairing Beyond the Seed Supports MicroRNA Targeting Specificity. Mol Cell, 2016 Oct 20:64:1-14. PMCID: PMC5074850

Mondol V, Ahn BC, Pasquinelli AE. Splicing remodels the let-7 primary microRNA to facilitate Drosha processing in Caenorhabditis elegans. RNA, 2015 21(8):1396-1403. PMCID: PMC4509930

Van Wynsberghe PM, Finnegan EF, Stark T, Angelus EP, Homan KE, Yeo GW, Pasquinelli AE. The Period protein homolog LIN-42 negatively regulates microRNA biogenesis in C. elegans. Dev Biol. 2014 Mar 31. pii: S0012-1606(14)00173-0. doi: 10.1016/j.ydbio.2014.03.017. PMCID: PMC4077587

Broughton JP, Pasquinelli AE.  Identifying Argonaute binding sites in Caenorhabditis elegans using iCLIP. Methods, 2013 Apr 10. S1046-2023(13)00102-3. PMCID: PMC3749250

Hunter SE, Finnegan EF, Zisoulis DG, Lovci MT, Melnik-Martinez KV, Yeo GW, Pasquinelli AE. Functional genomic analysis of the let-7 regulatory network in Caenorhabditis elegans. PLoS Genetics, 2013 Mar;9(3)e1003353. PMCID: PMC3597506

Kai, ZS, Finnegan EF, Huang S, Pasquinelli AE. Multiple cis-elements and trans-acting factors regulate dynamic spatio-temporal transcription of let-7 in Caenorhabditis elegans. Dev Biology, 2013 Feb 1;374(1):223-33. PMCID: PMC3548979

Zisoulis DG, Kai ZS, Chang RC, Pasquinelli AE. Auto-regulation of miRNA biogenesis by let-7 and Argonaute. Nature, 2012 Jun 28;486(7404):541-4. PMCID: PMC3387326

Massirer KB, Perez SG, Mondol V, Pasquinelli AE. The miR-35-41 family of microRNAs regulates RNAi sensitivity in Caenorhabditis elegans. PLoS Genetics. 2012 Mar;8(3):e1002536. PMCID: PMC3297572

Van Wynsberghe PM, Chan S-P, Slack FJ, Pasquinelli AE. Analysis of microRNA Expression and Function. Methods Cell Biol. 2011;106:219-52. PMID: 22118279 PMCID: PMC4314212

Zisoulis DG, Yeo GW, Pasquinelli AE. Comprehensive identification of miRNA target sites in live animals. Methods Mol Biol. 2011 732:169-85. DOI: 10.1007/978-1-61779-083-6_13

Van Wynsberghe PM, Kai ZS, Massirer KB, Burton VH, Yeo GW, Pasquinelli AE. LIN-28 co-transcriptionally binds primary let-7 to regulate miRNA maturation in Caenorhabditis elegans. Nat Struct Mol Biol. 2011 Mar;18(3):302-8. PMCID: PMC3077891

Bracht JR, Van Wynsberghe PM, Mondol V, Pasquinelli AE. Regulation of lin-4 miRNA Expression, Organismal Growth and Development by a Conserved RNA Binding Protein in C. elegans. Dev Biol. 2010 Dec 15;348(2):201-21. PMCID: PMC2982876

Zisoulis DG, Lovci MT, Wilbert ML, Hutt KR, Liang TY, Pasquinelli AE, Yeo GW. Comprehensive discovery of endogenous Argonaute binding sites in Caenorhabditis elegans. Nat Struct Mol Biol. 2010 17:173-9. PMCID: PMC2834287

Chendrimada TP, Finn KJ, Ji X, Baillant D, Gregory RI, Liebhaber SA, Pasquinelli AE, Shiekhattar R. MicroRNA silencing through RISC recruitment of eIF6.  Nature 2007 447:823-828. DOI: 10.1038/nature05841

Bagga S, Bracht J, Hunter S, Massirer K, Holtz J, Eachus R, Pasquinelli AE. Regulation by let-7 and lin-4miRNAs results in target mRNA degradation.  Cell. 2005 122:553-563. DOI: 10.1016/j.cell.2005.07.031

Bracht J, Hunter S, Eachus R, Weeks P, Pasquinelli AE.  Trans-splicing and polyadenylation of let-7microRNA primary transcripts. RNA. 2004 10:1586-1594. PMCID: PMC1370645

Pinning down miRNA targets in vivo